Isolation of Genomic DNA from Blood Using a Novel Filter-Based DNA Purification Technology | Zendy

Mark Baker | Zendy; Anna L. Mitchell | Zendy; C. Bridge | Zendy; S. Martin | Zendy; F. Holder | Zendy; Nishantha Pathirana | Zendy; Audrey W. Jarvis | Zendy; Nadeem Hafeez Butt | Zendy

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Isolation of Genomic DNA from Blood Using a Novel Filter-Based DNA Purification Technology

Author(s) -

Mark Baker,

Anna L. Mitchell,

C. Bridge,

S. Martin,

F. Holder,

Nishantha Pathirana,

Audrey W. Jarvis,

Nadeem Hafeez Butt

Publication year - 2001

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/01311pf01

Subject(s) - dna , genomic dna , chromatography , centrifugation , isolation (microbiology) , matrix (chemical analysis) , filter (signal processing) , dna extraction , computational biology , complex matrix , biology , chemistry , computer science , biochemistry , polymerase chain reaction , bioinformatics , gene , computer vision

A novel DNA purification technology is described that enables the purification of pure dsDNA from blood. When compared to existing DNA purification methods, the Whatman BioScience Purification System (WBPS) offers a fast and convenient way to recover high yields of DNA. WBPS is based on a unique filter system that entraps DNA within a matrix. This allows the process to be performed in a single unidirectional reaction vessel, reducing user interaction and multiple centrifugation steps.

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