Multicolor Imaging: The Important Question of Co-Localization | Zendy

Anna Smallcombe | Zendy

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Multicolor Imaging: The Important Question of Co-Localization

Author(s) -

Anna Smallcombe

Publication year - 2001

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/01306bt01

Subject(s) - colocalization , confocal microscopy , confocal , fluorescence microscope , microscopy , biophysics , biology , chemistry , fluorescence , microbiology and biotechnology , physics , optics

The use of specific fluorescent probes, combined with confocal or multiphoton microscopy and subsequent digital analysis of the images, has greatly improved the accuracy of co-localization of macromolecules in biological samples. This article addresses some of the caveats associated with the preparation and imaging of multilabeled samples and demonstrates the use of digital methods for measuring the extent of co-localization in cells.

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