Modification of an Automated Liquid-Handling System for Reagent-Jet, Nanoliter-Level Dispensing | Zendy

J. S. Hicks | Zendy; Brent W. Harker | Zendy; Kenneth L. Beattie | Zendy; Mitchel J. Doktycz | Zendy

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Modification of an Automated Liquid-Handling System for Reagent-Jet, Nanoliter-Level Dispensing

Author(s) -

J. S. Hicks,

Brent W. Harker,

Kenneth L. Beattie,

Mitchel J. Doktycz

Publication year - 2001

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/01304rr05

Subject(s) - volume (thermodynamics) , nozzle , nanotechnology , cabin pressurization , microfluidics , cartridge , computer science , jet (fluid) , reagent , materials science , process engineering , chemistry , computer hardware , engineering , mechanical engineering , aerospace engineering , physics , quantum mechanics

Reducing the scale of biochemical reactions is becoming commonplace. Examples include the screening of large libraries of chemical compounds or gene sequences. These applications demand the ability to transfer sub-microliter volumes of fluid. To this end, we have modified a Hamilton MICROLAB 2200 with high-speed solenoids and a liquid pressurization system to modulate volume delivery down to the nanoliter level. Additional modifications include the use of sapphire-tipped dispensing nozzles and a high-resolution substage to assist in the construction of DNA microarrays. Techniques for characterizing the dispensed volume are presented.

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