Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System | Zendy

Thomas A. Gardner | Zendy; Sungahn Ko | Zendy; Ling Yang | Zendy; John James Stewart Cadwell | Zendy; Leland W.K. Chung | Zendy; Chinghai Kao | Zendy

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Serum-Free Recombinant Production of Adenovirus Using a Hollow Fiber Capillary System

Author(s) -

Thomas A. Gardner,

Sungahn Ko,

Ling Yang,

John James Stewart Cadwell,

Leland W.K. Chung,

Chinghai Kao

Publication year - 2001

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/01302pf01

Subject(s) - fiber , recombinant dna , adenoviridae , capillary action , cell culture , viral vector , chromatography , biology , biomedical engineering , computational biology , chemistry , materials science , medicine , biochemistry , gene , genetics , organic chemistry , composite material

A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.

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