Spreadsheet-Based Program for the Analysis of DNA Methylation | Zendy

Ramaswamy Anbazhagan | Zendy; James G. Herman | Zendy; Kalyan Enika | Zendy; Edward Gabrielson | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Spreadsheet-Based Program for the Analysis of DNA Methylation

Author(s) -

Ramaswamy Anbazhagan,

James G. Herman,

Kalyan Enika,

Edward Gabrielson

Publication year - 2001

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/01301bc01

Subject(s) - cpg site , dna methylation , bisulfite sequencing , bisulfite , dna , methylation , biology , illumina methylation assay , cytosine , microbiology and biotechnology , promoter , differentially methylated regions , guanine , gene , computational biology , genetics , nucleotide , gene expression

Methylation of DNA in CpG dense regions of gene promoters (CpG islands) is important for transcriptional inactivation of selective genes in normal and neoplastic cells. Here, we present a spreadsheet-based program adapted from Microsoft Excel that is useful for identifying CpG islands and for assisting in the laboratory analysis of DNA methylation of these regions. Upon execution of the program, a customized workbook analyzes an entered DNA sequence for the total number and percentage cytosine and guanine nucleotides, the total number and percentage of CpG sites, and a CpG:GpC ratio. The program also displays the distribution of CpG sites in a visual format as well as in two different graphical formats. Finally, the program assists in laboratory studies of DNA methylation that employ bisulfite modification of DNA by displaying methylation-dependent effects of bisulfite treatment on DNA sequences.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research