Reusing Nylon Membranes for Radioactive Hybridizations | Zendy

David W. Thornbury | Zendy; Mark Farman | Zendy

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Reusing Nylon Membranes for Radioactive Hybridizations

Author(s) -

David W. Thornbury,

Mark Farman

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00296st05

Subject(s) - membrane , sodium hypochlorite , dna , covalent bond , reuse , dna–dna hybridization , in situ hybridization , chemistry , chromatography , biology , biochemistry , organic chemistry , gene , gene expression , ecology

We describe a procedure for recycling nylon hybridization membranes, enabling their repeated use for radioactive Southern hybridization analysis of different DNA samples. Following hybridization and probe removal, nylon membranes containing covalently linked DNAs were treated with 0.55% sodium hypochlorite. This destroyed the DNA, thereby preventing it from participating in further hybridization and enabling the membranes to be used subsequently for binding new DNA samples. With this procedure, we were able to reuse a single membrane as many as 13 times, with no detectable loss in signal. This method was shown to be effective for membranes supplied by three different manufacturers.

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