GFP and β-Galactosidase Transformation Vectors for Promoter/Enhancer Analysis in Drosophila | Zendy

Scott Barolo | Zendy; Lucy A. Carver | Zendy; James W. Posakony | Zendy

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GFP and β-Galactosidase Transformation Vectors for Promoter/Enhancer Analysis in Drosophila

Author(s) -

Scott Barolo,

Lucy A. Carver,

James W. Posakony

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00294bm10

Subject(s) - enhancer , transformation (genetics) , biology , green fluorescent protein , vector (molecular biology) , microbiology and biotechnology , genetics , recombinant dna , gene , gene expression

the fact that ECs are difficult to transfect, the success of this method is warranted for a wider range of cells with different origins. In fact, we have obtained similar results in temporally transfecting NIH 3T3 cells using this method (data not shown). In addition, this method of temporal transfection might have utility in reversing cellular abnormalities through genetic intervention of the temporally introduced gene.

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