Yeast Recombinase FLP Functions Effectively in Human Cells for Construction of Adenovirus Vectors | Zendy

Philip Ng | Zendy; Derek T. Cummings | Zendy; Carole Evelegh | Zendy; Frank L. Graham | Zendy

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Yeast Recombinase FLP Functions Effectively in Human Cells for Construction of Adenovirus Vectors

Author(s) -

Philip Ng,

Derek T. Cummings,

Carole Evelegh,

Frank L. Graham

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00293st04

Subject(s) - flp frt recombination , recombinase , recombinant dna , vector (molecular biology) , site specific recombination , plasmid , cre recombinase , biology , cre lox recombination , recombination , transfection , viral vector , computational biology , genetics , genome , virology , dna , genetic recombination , gene , transgene , genetically modified mouse

We have recently developed a high-efficiency method of constructing adenovirus vectors based on Cre-mediated recombination between two plasmids co-transfected into 293 cells. The simplicity and efficiency of this method should greatly expedite the construction of most recombinant vectors. However, this system would not be suitable for constructing vectors bearing loxP sites elsewhere in the genome because of undesirable Cre-mediated vector rearrangements. To address this, we have developed a similar system using FLP-mediated site-specific recombination for the construction of adenovirus vectors.

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