Streamlined Yeast Colorimetric Reporter Activity Assays Using Scanners and Plate Readers | Zendy

Ilya G. Serebriiskii | Zendy; Garabet G. Toby | Zendy; Erica A. Golemis | Zendy

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Streamlined Yeast Colorimetric Reporter Activity Assays Using Scanners and Plate Readers

Author(s) -

Ilya G. Serebriiskii,

Garabet G. Toby,

Erica A. Golemis

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00292st03

Subject(s) - biological system , two hybrid screening , yeast , hybrid system , computer science , scanner , dual (grammatical number) , computational biology , simple (philosophy) , biochemistry , biology , chemistry , artificial intelligence , machine learning , art , philosophy , literature , epistemology

Two-hybrid systems have become favored tools for detection and analysis of protein interactions because of their low cost and ease of use compared to biochemical or biophysical interaction technologies. It is possible to augment the utility of two-hybrid systems and derivative systems such as dual-bait two-hybrid systems by adapting strategies that speed the analysis of the relative strength of a series of protein-protein associations. This report describes two simple techniques that employ either a flatbed scanner or a plate reader to quantitate the activity of colorimetric reporters such as LacZ or GusA commonly used in two-hybrid approaches.

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