Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR ® Green II | Zendy

Cristina Battaglia | Zendy; Giuliana Salani | Zendy; Clarissa Consolandi | Zendy; Luigi Rossi Bernardi | Zendy; Gianluca De Bellis | Zendy

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Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR ^® Green II

Author(s) -

Cristina Battaglia,

Giuliana Salani,

Clarissa Consolandi,

Luigi Rossi Bernardi,

Gianluca De Bellis

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00291st01

Subject(s) - sybr green i , fluorophore , staining , fluorescence , dna , dna microarray , microbiology and biotechnology , biology , polymerase chain reaction , biochemistry , gene , genetics , optics , gene expression , physics

A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and homogeneity of each DNA spot of the array can thus be assessed. After this preliminary control, which may avoid further analytical steps that lead to the waste of precious biological samples, a fully reversible staining procedure is performed that produces an array ready for subsequent use.

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