Extraction of Nuclear Proteins with Increased DNA Binding Activity | Zendy

Beatrix A. Slomiany | Zendy; Margaret M. Kelly | Zendy; David T. Kurtz | Zendy

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Extraction of Nuclear Proteins with Increased DNA Binding Activity

Author(s) -

Beatrix A. Slomiany,

Margaret M. Kelly,

David T. Kurtz

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00285st08

Subject(s) - ammonium sulfate , extraction (chemistry) , dna , chemistry , chromatography , dna extraction , ammonium , biochemistry , transcription factor , gene , organic chemistry , polymerase chain reaction

We have developed a method for the rapid extraction of nuclear proteins from cultured cells. The ammonium sulfate method described here extracts larger quantities of proteins that retain DNA binding activity than the modified Dignam method and another popular method used for the extraction of transcription factors. The ammonium sulfate method is rapid and can be used to process a large number of samples for gel shift analysis.

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