Rapid Determination of Membrane Transport Parameters in Adherent Cells | Zendy

Karim A. Sharif | Zendy; I. David Goldman | Zendy

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Rapid Determination of Membrane Transport Parameters in Adherent Cells

Author(s) -

Karim A. Sharif,

I. David Goldman

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00285st06

Subject(s) - chinese hamster ovary cell , monolayer , membrane , chemosensitivity assay , substrate (aquarium) , chemistry , cell culture , biophysics , chromatography , membrane transport , in vitro , biochemistry , analytical chemistry (journal) , materials science , biology , ecology , genetics , receptor

Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials. The method is convenient, cost effective and requires no special apparatus. Initial uptake rates, steady-state and free substrate levels are demonstrated in ZR-75-1 breast cancer and Chinese hamster ovary cell lines using methotrexate, a model agent transported by the reduced folate carrier. The technique should be applicable to the study of the transport properties in a broad range of substrates and cells in monolayer culture.

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