DNA Extraction Method for Screening Yeast Clones by PCR | Zendy

Rinji Akada | Zendy; Tatsuhiko Murakane | Zendy; Yoshinori Nishizawa | Zendy

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DNA Extraction Method for Screening Yeast Clones by PCR

Author(s) -

Rinji Akada,

Tatsuhiko Murakane,

Yoshinori Nishizawa

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00284st02

Subject(s) - yeast , dna extraction , dna , biology , microbiology and biotechnology , polymerase chain reaction , extraction (chemistry) , chromatography , biochemistry , chemistry , gene

A simple procedure for isolating yeast DNA suitable for use as a template for PCR amplification is described. SDS treatment alone is sufficient for extraction of chromosomal DNA from yeast cells. Cells of a yeast colony are suspended in a small volume (about 20 μL) of a 0.25% SDS solution, mixed vigorously and centrifuged. The supernatant can be directly used as a template after dilution to give an SDS concentration of less than 0.01% in the final PCR mixture.

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