General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling | Zendy

Lily Y Wong | Zendy; Victor Belonogoff | Zendy; Victoria L. Boyd | Zendy; Nathan Hunkapiller | Zendy; Peter Casey | Zendy; Sueh-Ning Liew | Zendy; Katherine Lazaruk | Zendy; Susanne Baumhueter | Zendy

Open Access

General Method for HPLC Purification and Sequencing of Selected dsDNA Gene Fragments from Complex PCRs Generated during Gene Expression Profiling

Author(s) -

Lily Y Wong,

Victor Belonogoff,

Victoria L. Boyd,

Nathan Hunkapiller,

Peter Casey,

Sueh-Ning Liew,

Katherine Lazaruk,

Susanne Baumhueter

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00284rr07

Subject(s) - gene , biology , amplified fragment length polymorphism , dna , microbiology and biotechnology , gene expression profiling , gene expression , dna sequencing , genetics , population , demography , sociology , genetic diversity

Gene expression profiling using an AFLP-based technique generates a large number of gene fragments that require identification by sequencing. The DNA fragments vary in length from about 50-500 bp. Ion-pair reversed-phase HPLC can be used to purify selected double-stranded DNA fragments that represent differentially expressed genes. The gene fragments are sequenced directly after vacuum drying of the collected HPLC fractions.

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