Comparison of Two PCR Techniques Used in Amplification of Microdissected Plant Chromosomes from Rice and Wheat | Zendy

Yihua Zhou | Zendy; H Wang | Zendy; Jiali Wei | Zendy; Lihua Cui | Zendy; X. Deng | Zendy; X. Wang | Zendy; Ze Chen | Zendy

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Comparison of Two PCR Techniques Used in Amplification of Microdissected Plant Chromosomes from Rice and Wheat

Author(s) -

Yihua Zhou,

H Wang,

Jiali Wei,

Lihua Cui,

X. Deng,

X. Wang,

Ze Chen

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00284rr06

Subject(s) - biology , microbiology and biotechnology , meiosis , chromosome , genetics , polymerase chain reaction , gene

Linker adaptor-mediated PCR (LAM-PCR) and degenerate oligonucleotide primed PCR (DOP-PCR) are major ways to generate chromosome-specific libraries. In this study, these two PCR techniques were used to amplify the microdissected mitotic and meiotic chromosomes from rice and wheat. The results of amplifications were compared, and the advantages and limitations between the two techniques are presented.

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