Detection of Eukaryotic cDNA in Differential Display Is Enhanced by the Addition of E. coli RNA | Zendy

Heather J. Melichar | Zendy; Irene Bosch | Zendy; G Molnar | Zendy; Linfeng Huang | Zendy; Arthur B. Pardee | Zendy

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Detection of Eukaryotic cDNA in Differential Display Is Enhanced by the Addition of E. coli RNA

Author(s) -

Heather J. Melichar,

Irene Bosch,

G Molnar,

Linfeng Huang,

Arthur B. Pardee

Publication year - 2000

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/00281st01

Subject(s) - complementary dna , rna , differential display , reverse transcriptase , biology , transcription (linguistics) , microbiology and biotechnology , gene , escherichia coli , messenger rna , gene expression , genetics , linguistics , philosophy

We describe a method to enhance the sensitivity of eukaryotic cDNA detection in differential display (DD). Typically, DD protocols require between 200 and 500 ng RNA for each reverse transcription reaction. The addition of Escherichia coli RNA before reverse transcription of eukaryotic RNA increases the detection of DD patterns more than tenfold. The method broadens the applicability of DD and allows the identification of genes that are differentially expressed when the amount of eukaryotic RNA is limited.

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