BODIPY-Based Dye for No-Wash Live-Cell Staining and Imaging | Zendy

Alexey A. Pakhomov | Zendy; I. E. Deyev | Zendy; N. M. Ratnikova | Zendy; С. П. Чумаков | Zendy; Veronika B. Mironiuk | Zendy; Yuriy N. Kovich | Zendy; Aziz M. Muzafarov | Zendy; Vladimir I. Martynov | Zendy

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BODIPY-Based Dye for No-Wash Live-Cell Staining and Imaging

Author(s) -

Alexey A. Pakhomov,

I. E. Deyev,

N. M. Ratnikova,

С. П. Чумаков,

Veronika B. Mironiuk,

Yuriy N. Kovich,

Aziz M. Muzafarov,

Vladimir I. Martynov

Publication year - 2017

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114577

Subject(s) - bodipy , solvatochromism , staining , fluorescence , flow cytometry , photochemistry , biophysics , chemistry , quenching (fluorescence) , derivative (finance) , fluorescence microscope , fluorescein , microbiology and biotechnology , biology , optics , pathology , medicine , physics , financial economics , economics

In nonpolar solvents, hydrophobic organic fluorophores often show bright fluorescence, whereas in polar media, they usually suffer from aggregation-caused quenching (ACQ). Here, we harnessed this solvatochromic behavior of a 1,3,5,7-tetramethyl-BODIPY derivative for cell staining and applied it to live-cell imaging and flow cytometry. As opposed to commercially available dyes, this BODIPY derivative showed excellent contrast immediately after staining and did not require any wash-off.

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