Open AccessSingle-Step PCR-Based Genetic Sex Determination of Rat Tissues and Cells
Author(s) -
Pramod Dhakal,
Michael J. Soares
Publication year - 2017
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000114548
Subject(s) - biology , gene , computational biology , genome , genetics , function (biology) , genome editing , in vivo
The advent of genome editing strategies has expanded the range of animal models available for gene manipulation and renewed research interest in the rat. Gender is a key variable for in vivo gene function analyses. Here, we present a simple PCR-based method to determine genetic sex in the rat.
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