Fin clipping and genotyping embryonic zebrafish at 3 days post-fertilization | Zendy

Robert N. Wilkinson | Zendy; Stone Elworthy | Zendy; Philip W. Ingham | Zendy; Fredericus J. M. van Eeden | Zendy

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Fin clipping and genotyping embryonic zebrafish at 3 days post-fertilization

Author(s) -

Robert N. Wilkinson,

Stone Elworthy,

Philip W. Ingham,

Fredericus J. M. van Eeden

Publication year - 2017

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114509

Subject(s) - genotyping , zebrafish , biology , human fertilization , embryo , fish fin , embryonic stem cell , genotype , embryogenesis , genetics , andrology , anatomy , fishery , gene , fish <actinopterygii> , medicine

Protocol Summary Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.

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