A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins | Zendy

Akifumi Nakagawa | Zendy; Kate Von Alt | Zendy; Keith D. Lillemoe | Zendy; Carlos Fernández-del Castillo | Zendy; Andrew L. Warshaw | Zendy; Andrew S. Liss | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A method for fixing and paraffin embedding tissue to retain the natural fluorescence of reporter proteins

Author(s) -

Akifumi Nakagawa,

Kate Von Alt,

Keith D. Lillemoe,

Carlos Fernández-del Castillo,

Andrew L. Warshaw,

Andrew S. Liss

Publication year - 2017

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114505

Subject(s) - fluorescence , green fluorescent protein , reporter gene , biology , cytoplasm , microbiology and biotechnology , protein subcellular localization prediction , chemistry , biochemistry , gene , gene expression , physics , quantum mechanics

Protocol Summary Here we present an ethanol fixation protocol that allows for the direct visualization of the natural fluorescence of reporter proteins while maintaining excellent tissue histology. We demonstrate the utility of this method for visualizing green and red fluorescent proteins in a wide range of murine tissues using both cytoplasmic and membrane-localized fluorescent reporter proteins. Tissues fixed by this method also allow for immunohistochemical studies, providing a single method to visualize the natural fluorescence of reporter proteins with subsequent detection of cellular proteins.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research