RNA extraction from sorted neuronal subtypes | Zendy

Alka Saxena | Zendy; Akiko Wagatsuma | Zendy; Yukihiko Noro | Zendy; Takenobu Kuji | Zendy; Atsuko Asaka-Oba | Zendy; Akira Watahiki | Zendy; Cecile Gurnot | Zendy; Michela Fagiolini | Zendy; Takao K. Hensch | Zendy; Piero Carninci | Zendy

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RNA extraction from sorted neuronal subtypes

Author(s) -

Alka Saxena,

Akiko Wagatsuma,

Yukihiko Noro,

Takenobu Kuji,

Atsuko Asaka-Oba,

Akira Watahiki,

Cecile Gurnot,

Michela Fagiolini,

Takao K. Hensch,

Piero Carninci

Publication year - 2017

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114499

Subject(s) - rna extraction , rna , transcriptome , cell sorting , biology , cell , microbiology and biotechnology , extracellular matrix , dna , computational biology , gene expression , genetics , gene

Protocol Summary Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq.

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