dsDNA-coated quantum dots | Zendy

Xing Ming | Zendy; Huaibin Shen | Zendy; Wei Zhao | Zendy; Yanfei Liu | Zendy; Yingda Du | Zendy; Zhenxiang Yu | Zendy; Xia Chen | Zendy

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dsDNA-coated quantum dots

Author(s) -

Xing Ming,

Huaibin Shen,

Wei Zhao,

Yanfei Liu,

Yingda Du,

Zhenxiang Yu,

Xia Chen

Publication year - 2017

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114498

Subject(s) - genetics , quantum dot , computational biology , biology , dna , physics , optoelectronics

Protocol Summary We developed a versatile QD label by modifying dsDNA with biotin and thiol groups at opposite ends and attaching it to quantum dots via a metal-thiol bond. These dsDNA-coated QDs fluorescently label their targets through biotin-streptavidin binding and show excellent histological results when used to detect biotin-labeled chromosome probes. The dsDNA coating also circumvented the common problems of aggregation and steric hindrance that occur with other QD probes.

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