High-Throughput Sequencing of Forensic Genetic Samples Using Punches of FTA Cards with Buccal Swabs | Zendy

MarieLouise Kampmann | Zendy; Anders Buchard | Zendy; Claus B⊘rsting | Zendy; Niels Morling | Zendy

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High-Throughput Sequencing of Forensic Genetic Samples Using Punches of FTA Cards with Buccal Swabs

Author(s) -

MarieLouise Kampmann,

Anders Buchard,

Claus B⊘rsting,

Niels Morling

Publication year - 2016

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114453

Subject(s) - buccal swab , massive parallel sequencing , ion semiconductor sequencing , dna sequencing , buccal administration , biology , polymerase chain reaction , forensic genetics , dna , dna profiling , computational biology , genetics , microsatellite , bioinformatics , gene , allele

Here, we demonstrate that punches from buccal swab samples preserved on FTA cards can be used for high-throughput DNA sequencing, also known as massively parallel sequencing (MPS). We typed 44 reference samples with the HID-Ion AmpliSeq Identity Panel using washed 1.2 mm punches from FTA cards with buccal swabs and compared the results with those obtained with DNA extracted using the EZ1 DNA Investigator Kit. Concordant profiles were obtained for all samples. Our protocol includes simple punch, wash, and PCR steps, reducing cost and hands-on time in the laboratory. Furthermore, it facilitates automation of DNA sequencing.

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