Development of a bench-top extra-cleanroom for DNA amplification | Zendy

Hirokazu Takahashi | Zendy; Takahiro Satoh | Zendy; Hiroko Kanahara | Zendy; Yuji Kubota | Zendy; Tamaki Hirose | Zendy; Hiroyuki Yamazaki | Zendy; Kimiko Yamamoto | Zendy; Yoshiko Okamura | Zendy; Taketo Suzuki | Zendy; Toshiro Kobori | Zendy

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Development of a bench-top extra-cleanroom for DNA amplification

Author(s) -

Hirokazu Takahashi,

Takahiro Satoh,

Hiroko Kanahara,

Yuji Kubota,

Tamaki Hirose,

Hiroyuki Yamazaki,

Kimiko Yamamoto,

Yoshiko Okamura,

Taketo Suzuki,

Toshiro Kobori

Publication year - 2016

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114433

Subject(s) - cleanroom , contamination , human decontamination , environmental science , nanotechnology , engineering , waste management , materials science , biology , ecology

Prevention of airborne contamination has become an important factor in biotechnology; however, conventional laminar-airflow cabinets (LAF-cabinets) are no longer sufficient as a countermeasure against nano-sized airborne contaminants in the laboratory. Here we present a bench-top extra-cleanroom classified as ISO-1 that can prevent contamination from airborne nanoparticles. This bench-top extra-cleanroom consists of a novel clean-zone-creating system that is equipped with nanofibrous, nonwoven filters. In addition, the cleanroom is also equipped with an ionizer to prevent plasticware from collecting dust by electrostatic charge attraction. This combination of features allows the cleanroom to prevent DNA contamination derived from airborne nanoparticles. Our extra-cleanroom with ionizer could be useful in various areas of biotechnology that are easily affected by airborne contaminants.

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