Comparison of Fluorescent Intercalating Dyes for Quantitative Loop-Mediated Isothermal Amplification (qLAMP) | Zendy

Igor P. Oscorbin | Zendy; E. A. Belousova | Zendy; А. И. Закабунин | Zendy; U. A. Boyarskikh | Zendy; М. Л. Филипенко | Zendy

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Comparison of Fluorescent Intercalating Dyes for Quantitative Loop-Mediated Isothermal Amplification (qLAMP)

Author(s) -

Igor P. Oscorbin,

E. A. Belousova,

А. И. Закабунин,

U. A. Boyarskikh,

М. Л. Филипенко

Publication year - 2016

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114432

Subject(s) - loop mediated isothermal amplification , fluorescence , intercalation (chemistry) , isothermal process , loop (graph theory) , chemistry , microbiology and biotechnology , quantitative analysis (chemistry) , biophysics , dna , chromatography , biology , biochemistry , thermodynamics , optics , physics , inorganic chemistry , mathematics , combinatorics

Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the environment. Here we present a comparative study of the performance of six fluorescent intercalating dyes—SYTO-9, SYTO-13, SYTO-82, SYBR Green I, SYBR Gold, EvaGreen—in three different qLAMP model systems. SYTO-9 and SYTO-82, which had the best results, were used for additional enzyme and template titration studies. SYTO-82 demonstrated the best combination of time-to-threshold (T t ) and signal-to-noise ratio (SNR).

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