Bioanalyzer Chips can be Used Interchangeably For Many Analyses Of DNA or RNA | Zendy

Jessica L. Davies | Zendy; Tom Denyer | Zendy; James Hadfield | Zendy

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Bioanalyzer Chips can be Used Interchangeably For Many Analyses Of DNA or RNA

Author(s) -

Jessica L. Davies,

Tom Denyer,

James Hadfield

Publication year - 2016

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114403

Subject(s) - rna , dna , nucleic acid , microfluidics , economic shortage , dna sequencing , microfluidic chip , microbiology and biotechnology , computational biology , biology , chemistry , nanotechnology , biochemistry , gene , materials science , linguistics , philosophy , government (linguistics)

The Agilent 2100 Bioanalyzer enables small-scale gel electrophoretic separation of nucleic acids on a microfluidic chip; however, a shortage of chips and an excess of reagents are common issues. Here we explore the compatibility of two commonly used Bioanalyzer reagents with three Bioanalyzer chip types. Microfluidic electrophoretic separation of DNA and RNA using DNA High Sensitivity and RNA 6000 Nano reagents, respectively, was successfully performed on multiple chip types following the assay-specific protocols. For RNA quality and next-generation sequencing (NGS) library length estimation, the Bioanalyzer chips we tested can be used interchangeably. These findings will be valuable for any laboratory using the Agilent Bioanalyzer in a shared facility.

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