A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids | Zendy

Frank Eckerdt | Zendy; Angel A. Alvarez | Zendy; Jonathan B. Bell | Zendy; Constadina Arvanitis | Zendy; Asneha Iqbal | Zendy; Ahmet Arslan | Zendy; Bo Hu | Zendy; Shi-Yuan Cheng | Zendy; Stewart Goldman | Zendy; Leonidas C. Platanias | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A simple, low-cost staining method for rapid-throughput analysis of tumor spheroids

Author(s) -

Frank Eckerdt,

Angel A. Alvarez,

Jonathan B. Bell,

Constadina Arvanitis,

Asneha Iqbal,

Ahmet Arslan,

Bo Hu,

Shi-Yuan Cheng,

Stewart Goldman,

Leonidas C. Platanias

Publication year - 2016

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114372

Subject(s) - spheroid , neurosphere , acridine orange , staining , biology , high throughput screening , cell culture , chemistry , cancer research , bioinformatics , biochemistry , cellular differentiation , genetics , adult stem cell , gene

Tumor spheroids are becoming an important tool for the investigation of cancer stem cell (CSC) function in tumors; thus, low-cost and high-throughput methods for drug screening of tumor spheroids are needed. Using neurospheres as non-adherent three-dimensional (3-D) cultures, we developed a simple, low-cost acridine orange (AO)-based method that allows for rapid analysis of live neurospheres by fluorescence microscopy in a 96-well format. This assay measures the cross-section area of a spheroid, which corresponds to cell viability. Our novel method allows rapid screening of a panel of anti-proliferative drugs to assess inhibitory effects on the growth of cancer stem cells in 3-D cultures.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research