High-Throughput Detection of DNA Double-Strand Breaks Using Image Cytometry | Zendy

Tyler L. Fowler | Zendy; Alison M. Bailey | Zendy; Bryan Bednarz | Zendy; Randall J. Kimple | Zendy

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High-Throughput Detection of DNA Double-Strand Breaks Using Image Cytometry

Author(s) -

Tyler L. Fowler,

Alison M. Bailey,

Bryan Bednarz,

Randall J. Kimple

Publication year - 2015

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114248

Subject(s) - immunofluorescence , dna , throughput , double strand , cytometry , flow cytometry , microbiology and biotechnology , computational biology , biology , dna damage , computer science , genetics , antibody , telecommunications , wireless

Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated image cytometry assessment of cell average γH2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method for γH2AX analysis.

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