Investigation of Membrane Protein—protein Interactions Using Correlative FRET-PLA | Zendy

Daniel Ivanusic | Zendy; Magdalena Eschricht | Zendy; Joachim Denner | Zendy

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Investigation of Membrane Protein—protein Interactions Using Correlative FRET-PLA

Author(s) -

Daniel Ivanusic,

Magdalena Eschricht,

Joachim Denner

Publication year - 2014

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114215

Subject(s) - förster resonance energy transfer , correlative , proximity ligation assay , immunoprecipitation , protein–protein interaction , in situ , biophysics , membrane protein , membrane , chemistry , fluorescence , biochemistry , biology , gene , philosophy , linguistics , physics , quantum mechanics , receptor , organic chemistry

Fluorescence resonance energy transfer (FRET) analysis and the recently developed proximity ligation assay (PLA) are widely used to study protein-protein interactions in situ. We have developed correlative FRET-PLA to monitor interactions between membrane proteins that frequently cause problems in confirmatory co-immunoprecipitation assays. Correlative FRET-PLA is particularly aimed at delivering robust and reliable results and is useful for investigating protein-protein interactions.

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