Open AccessA method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies
Author(s) -
Robert N. Wilkinson,
Stone Elworthy,
Philip W. Ingham,
Fredericus J.M. van Eeden
Publication year - 2013
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000114116
Subject(s) - genotyping , zebrafish , biology , embryo , throughput , genotype , polymerase chain reaction , genetics , computational biology , gene , computer science , telecommunications , wireless
Here we describe a method for high-throughput genotyping of live larval zebrafish as early as 72 h post-fertilization (hpf). Importantly, this technique allows rapid and cost-effective PCR-based genotyping from very small fin biopsies, which regenerate as the embryo develops, thereby allowing researchers to select embryos with desired genotypes to be raised to adulthood.