Open AccessCorrelative light microscopy for high-content screening
Author(s) -
Benjamin Flottmann,
Manuel Gunkel,
Tautvydas Lisauskas,
Mike Heilemann,
Vytaute Starkuviene,
Jürgen Reymann,
Holger Erfle
Publication year - 2013
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000114099
Subject(s) - microscopy , confocal microscopy , microscope , resolution (logic) , confocal , light sheet fluorescence microscopy , correlative , throughput , super resolution microscopy , optics , computer science , scanning confocal electron microscopy , artificial intelligence , physics , telecommunications , linguistics , philosophy , wireless
High-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution microscopy. We demonstrate the potential of this approach for scalable experiments. The workflow consists of a robust approach for selecting cells of interest on a wide-field screening microscope at low resolution and subsequently re-localizing those cells with micrometer precision for confocal and super-resolution imaging. As a case study, we visualized and quantified cis- and trans-Golgi markers at increasing resolution.