Mild Hypothermia Inhibits Differentiation of Human Embryonic and Induced Pluripotent Stem Cells | Zendy

Glenn S. Belinsky | Zendy; Srdjan D. Antic | Zendy

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Mild Hypothermia Inhibits Differentiation of Human Embryonic and Induced Pluripotent Stem Cells

Author(s) -

Glenn S. Belinsky,

Srdjan D. Antic

Publication year - 2013

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000114065

Subject(s) - induced pluripotent stem cell , embryonic stem cell , microbiology and biotechnology , biology , kosr , cellular differentiation , stem cell , gene , genetics

Culture of pluripotent stem cells at 35°C strikingly reduces unwanted spontaneous differentiation during hESC and iPSC maintenance compared with 37°C. Growth at 35°C did not affect expression of pluripotency mRNAs nor induce expression of cold-inducible genes. Colony size was somewhat reduced at 35°C. Thus, growth at 35°C is a convenient, simple method to reduce the labor of removing spontaneously differentiated colonies when maintaining pluripotent cells.

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