In-solution Staining and Arraying Method for the Immunofluorescence Detection of γH2AX Foci Optimized for Clinical Applications | Zendy

Pegah Johansson | Zendy; Aida Muslimović | Zendy; Ragnar Hultborn | Zendy; Erik Fernström | Zendy; Ola Hammarsten | Zendy

Open Access

In-solution Staining and Arraying Method for the Immunofluorescence Detection of γH2AX Foci Optimized for Clinical Applications

Author(s) -

Pegah Johansson,

Aida Muslimović,

Ragnar Hultborn,

Erik Fernström,

Ola Hammarsten

Publication year - 2011

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000113738

Subject(s) - immunofluorescence , staining , dna , microbiology and biotechnology , ionizing radiation , indirect immunofluorescence , microscopy , pathology , biology , irradiation , medicine , antibody , physics , immunology , biochemistry , nuclear physics

Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted and fixed onto microscope slides. Our modified method allows arraying of 16 patient samples/slide ready for foci counting in 2 h and demonstrated reliably detection of γH2AX foci in mononuclear cells prepared from patients who had undergone radiation therapy.

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