A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion and Gateway technology | Zendy

Takeya Morozumi | Zendy; Daisuke Toki | Zendy; Tomoko Eguchi-Ogawa | Zendy; Hirohide Uenishi | Zendy

Open Access

A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion and Gateway technology

Author(s) -

Takeya Morozumi,

Daisuke Toki,

Tomoko Eguchi-Ogawa,

Hirohide Uenishi

Publication year - 2011

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000113737

Subject(s) - shotgun sequencing , transposable element , complementary dna , biology , dna sequencing , computational biology , genetics , single cell sequencing , massive parallel sequencing , illumina dye sequencing , deep sequencing , shotgun , exome sequencing , dna , gene , mutation , genome

Large-scale cDNA-sequencing projects require an efficient strategy for mass sequencing. Here we describe a method for sequencing pooled cDNA clones using a combination of transposon insertion and Gateway technology. Our method reduces the number of shotgun clones that are unsuitable for reconstruction of cDNA sequences, and has the advantage of reducing the total costs of the sequencing project.

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