Immunoprecipitation and the High Sensitivity Protein 250 Assay | Zendy

Christian Wenz | Zendy; Andreas Rüfer | Zendy

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Immunoprecipitation and the High Sensitivity Protein 250 Assay

Author(s) -

Christian Wenz,

Andreas Rüfer

Publication year - 2010

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000113404

Subject(s) - immunoprecipitation , blot , protein detection , antibody , sensitivity (control systems) , protein expression , reagent , microbiology and biotechnology , assay sensitivity , computational biology , chemistry , chromatography , biology , biochemistry , gene , nanotechnology , immunology , medicine , materials science , alternative medicine , pathology , electronic engineering , engineering

A new method for the targeted analysis of proteins is presented, which combines the specificity of immunopre cipitation with the sensitivity of protein detection on microchips using the High Sensitivity Protein 250 assay for the Agilent 2100 Bioanalyzer. As as an alternative to Western blotting, this method is valuable for researchers engaged, for example, in protein expression and purification in pharma, biotech, or academic labs. Advantages of this new method in comparison to Western blotting are: • More reliable results: higher specificity and sensitivity • Better accuracy and precision: less manual steps and direct availability of quantitative data • Increased productivity: 3 hours versus 1 day analysis time • Lower spending for antibodies: 10× less primary and no secondary antibody are needed • Lower reagent consumption: environmentally friendly process

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