A lens-specific co-injection marker for medaka transgenesis | Zendy

Pavel Vopálenský | Zendy; Jana Růžičková | Zendy; Barbora Pavlů | Zendy; Zbyněk Kozmík | Zendy

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A lens-specific co-injection marker for medaka transgenesis

Author(s) -

Pavel Vopálenský,

Jana Růžičková,

Barbora Pavlů,

Zbyněk Kozmík

Publication year - 2010

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000113368

Subject(s) - transgenesis , transgene , biology , heterologous , reporter gene , germline , marker gene , microbiology and biotechnology , genetically modified mouse , cell sorting , gene , genetics , gene expression , cell , reproductive technology , embryogenesis

Recent advances in generating transgenic fish have improved the efficiency of germline transmission and enabled the generation of large numbers of transgenic animals. A suitable co-injection marker may help facilitate the preselection of transgenic embryos. For this purpose, a lens-specific marker appears to be a suitable candidate since the lens is a well-defined tissue that is easily accessible for examination of reporter gene expression. We constructed reporter vectors including the mouse gamma-F crystallin (mgammaF-Cry) promoter, which drives high levels of lens-specific heterologous expression of the reporter gene and thereby enables easy sorting of transgenic fish.

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