Simplified DGS procedure for large-scale genome structural study | Zendy

Yong-Chul Jung | Zendy; Jia Xu | Zendy; Jun Chen | Zendy; Yeong C. Kim | Zendy; David J. Winchester | Zendy; San Ming Wang | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Simplified DGS procedure for large-scale genome structural study

Author(s) -

Yong-Chul Jung,

Jia Xu,

Jun Chen,

Yeong C. Kim,

David J. Winchester,

San Ming Wang

Publication year - 2009

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000113294

Subject(s) - genome , computational biology , biology , dna sequencing , dna sequencer , reference genome , genetics , restriction enzyme , sequence (biology) , dna , gene

Ditag genome scanning (DGS) uses next-generation DNA sequencing to sequence the ends of ditag fragments produced by restriction enzymes. These sequences are compared to known genome sequences to determine their structure. In order to use DGS for large-scale genome structural studies, we have substantially revised the original protocol by replacing the in vivo genomic DNA cloning with in vitro adaptor ligation, eliminating the ditag concatemerization steps, and replacing the 454 sequencer with Solexa or SOLiD sequencers for ditag sequence collection. This revised protocol further increases genome coverage and resolution and allows DGS to be used to analyze multiple genomes simultaneously.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research