Open AccessEnhanced amplification of GC-rich DNA with two organic reagents
Author(s) -
Zhizhou Zhang,
Xia Yang,
Liangyu Meng,
Fang Liu,
Cenchao Shen,
Wenjuan Yang
Publication year - 2009
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000113203
Subject(s) - dna , polymerase chain reaction , biology , genetics , computational biology , chemistry , microbiology and biotechnology , gene
Amplification of GC-rich DNA sequences is still a difficult task worldwide. Two frequently seen and inexpensive reagents-ethylene glycol and 1,2-propanediol-were found to be more effective than betaine in the amplification of 104 randomly selected GC-rich human DNA sequences with GC contents of 60-80% and lengths of 700-800 bp.
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