Open AccessAnalysis of insoluble proteins
Author(s) -
Sarah Trimpin,
Bill Brizzard
Publication year - 2009
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000113135
Subject(s) - proteomics , computational biology , membrane protein , mass spectrometry , chemistry , human proteins , genome , biology , biochemistry , chromatography , membrane , gene
The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20–30% of the proteins encoded by the human genome. Chemical analysis on an individual basis is often required and is laborious and time consuming. This review presents an overview of methods for purification of expressed proteins using fusion tags as well as methods for analysis of insoluble proteins by mass spectrometry with a goal of achieving high-throughput analysis.