A device for automated hydrodynamic shearing of genomic DNA | Zendy

Aric Joneja | Zendy; Xiaohua Huang | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A device for automated hydrodynamic shearing of genomic DNA

Author(s) -

Aric Joneja,

Xiaohua Huang

Publication year - 2009

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000113123

Subject(s) - shearing (physics) , body orifice , genomic dna , microfluidics , dna , materials science , biology , nanotechnology , genetics , anatomy , composite material

We describe a device for automated fragmentation of genomic DNA by hydrodynamic shearing using a filter screen with uniform pores. Human genomic DNA can be fragmented reproducibly to a range of 2000-12,000 bp by using various fluid flow rates and screens with 0.5-10-microm pores. The utilization of disposable screens eliminates sample cross-contamination and the tendency of device clogging, commonly encountered in single-orifice shearing devices.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research