Improvements to Bead-Based Oligonucleotide Ligation SNP Genotyping Assays | Zendy

Shan Bruse | Zendy; Michael Moreau | Zendy; Marco A. Azaro | Zendy; Ray A. Zimmerman | Zendy; Linda M. Brzustowicz | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Improvements to Bead-Based Oligonucleotide Ligation SNP Genotyping Assays

Author(s) -

Shan Bruse,

Michael Moreau,

Marco A. Azaro,

Ray A. Zimmerman,

Linda M. Brzustowicz

Publication year - 2008

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112960

Subject(s) - oligonucleotide , snp genotyping , genotyping , ligation , bead , multiplex , pyrosequencing , biology , computational biology , snp , microbiology and biotechnology , single nucleotide polymorphism , dna , genetics , genotype , gene , materials science , composite material

We describe a bead-based, multiplexed, oligonucleotide ligation assay (OLA) performed on the Luminex flow cytometer. Differences between this method and those previously reported include the use of far fewer beads and the use of a universal oligonucleotide for signal detection. These innovations serve to significantly reduce the cost of the assay, while maintaining robustness and accuracy. Comparisons are made between the Luminex OLA and both pyrosequencing and direct sequencing. Experiments to assess conversion rates, call rates, and concordance across technical replicates are also presented.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research