A Quantitative Real-Time PCR Method for Absolute Telomere Length | Zendy

Nathan J. O’Callaghan | Zendy; Varinderpal S. Dhillon | Zendy; Philip Thomas | Zendy; Michael Fenech | Zendy

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A Quantitative Real-Time PCR Method for Absolute Telomere Length

Author(s) -

Nathan J. O’Callaghan,

Varinderpal S. Dhillon,

Philip Thomas,

Michael Fenech

Publication year - 2008

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112761

Subject(s) - telomere , real time polymerase chain reaction , biology , microbiology and biotechnology , restriction fragment , genetics , dna , gene

Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our method uses an oligomer standard that can be used to generate absolute telomere length values rather than relative quantification. We demonstrate a strong correlation between this improved method and the "gold standard" of telomere length measurement-terminal restriction fragment analysis (TRF) by Southern hybridization. The capability to generate absolute telomere length values should allow a more direct comparison of results between experiments within and between laboratories.

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