Amplifying Small Amounts of Tumor DNA Allows Detection of DNA Copy Number Aberrations with Array-CGH | Zendy

Lars Prestegarden | Zendy; Anjan Misra | Zendy; Marcus Ware | Zendy; Ru-Fang Yeh | Zendy; Rolf Bjerkvig | Zendy; Burt G. Feuerstein | Zendy

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Amplifying Small Amounts of Tumor DNA Allows Detection of DNA Copy Number Aberrations with Array-CGH

Author(s) -

Lars Prestegarden,

Anjan Misra,

Marcus Ware,

Ru-Fang Yeh,

Rolf Bjerkvig,

Burt G. Feuerstein

Publication year - 2008

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112760

Subject(s) - comparative genomic hybridization , biology , genomic dna , dna , copy number analysis , copy number variation , genetics , computational biology , microbiology and biotechnology , genome , gene

Array comparative genomic hybridization (aCGH) is a powerful tool to detect relative DNA copy number at a resolution limited only by the coverage of bacterial artificial chromosomes (BACs) used to print the genomic array. The amount of DNA needed to perform a reliable aCGH analysis has been a limiting factor, especially on minute tissue samples where limited DNA is available. Here we report a simple, highly sensitive and reliable aCGH method to analyze samples of no more than 1 ng genomic DNA. The speed and simplicity of the technique are ideal for studies on small clinical samples such as needle biopsies.

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