Ultrasound in Phage Display: A New Approach to Nonspecific Elution | Zendy

Mojca Lunder | Zendy; Tomaž Bratkovič | Zendy; U. Urleb | Zendy; Samo Kreft | Zendy; Borut Štrukelj | Zendy

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Ultrasound in Phage Display: A New Approach to Nonspecific Elution

Author(s) -

Mojca Lunder,

Tomaž Bratkovič,

U. Urleb,

Samo Kreft,

Borut Štrukelj

Publication year - 2008

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112759

Subject(s) - elution , phage display , computational biology , peptide , peptide library , competitive binding , chemistry , selection (genetic algorithm) , biology , chromatography , biochemistry , peptide sequence , computer science , artificial intelligence , gene , receptor

Libraries of phage-displayed random peptides are routinely used to identify target-binding peptides. Phages are commonly eluted in a nonspecific manner, especially if there are no available ligands of the particular target to use as competitors. However, the present study clearly demonstrates that nonspecific elution is not always able to break peptide-target interactions. To circumvent this we have developed an improved nonspecific elution strategy that uses ultrasound to release target-bound phages and enables selection of high-affinity clones in a single step.

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