The establishment of in vivo-like tissue culture conditions in ThinCert™ tissue culture products | Zendy

Sven Mühlfriedel | Zendy; G. Knebel | Zendy

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The establishment of in vivo-like tissue culture conditions in ThinCert™ tissue culture products

Author(s) -

Sven Mühlfriedel,

G. Knebel

Publication year - 2007

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112701

Subject(s) - tissue culture , in vivo , cell culture , tissue engineering , in vitro , microbiology and biotechnology , biology , function (biology) , organ culture , biochemistry , genetics

Greiner Bio-One GmbH, Frickenhausen, GermanyINTRODUCTIONFor many years animal experimentation provided the singular means to access intact and living tissue for biolog-ical and pharmaceutical research. This situation changed with the introduction of cell culture methods that allowed tissue reconstruction from individual cells in vitro. Such approaches typically involve the use of porous membrane supports and/or three-dimensional collagen gels to re-create in vivo-like growth in cell culture environments

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