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Greiner Bio-One GmbH, Frickenhausen, GermanyINTRODUCTIONFor many years animal experimentation provided the singular means to access intact and living tissue for biolog-ical and pharmaceutical research. This situation changed with the introduction of cell culture methods that allowed tissue reconstruction from individual cells in vitro. Such approaches typically involve the use of porous membrane supports and/or three-dimensional collagen gels to re-create in vivo-like growth in cell culture environments

The establishment of in vivo-like tissue culture conditions in ThinCert™ tissue culture products