Real-Time Viability Assay Based on 51 Cr Retention in Adherent Cells | Zendy

Lina Vennström | Zendy; Camilla Bysell | Zendy; Hanna Björkelund | Zendy; Hans Lundqvist | Zendy; Karl Andersson | Zendy

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Real-Time Viability Assay Based on ⁵¹ Cr Retention in Adherent Cells

Author(s) -

Lina Vennström,

Camilla Bysell,

Hanna Björkelund,

Hans Lundqvist,

Karl Andersson

Publication year - 2008

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112688

Subject(s) - viability assay , microbiology and biotechnology , chemistry , cell culture , biology , in vitro , biochemistry , genetics

The chromium (51Cr) release assay has been widely used for viability measurements, even though it has major disadvantages such as high manual workload and poor time resolution. By the use of LigandTracer 51Cr release viability measurements on adherent cells can be significantly simplified and improved. LigandTracer enables a time-resolved detection of 5SCr in target cells, with the result that the effect of toxic material is updated continuously throughout the experiment. Here we explain the principle behind this novel real-time viability assay and show viability curves for known toxic compounds on A431 and U343MGaCl2:6 cell lines.

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