A Microfluidic System for Dynamic Yeast Cell Imaging | Zendy

Philip Lee | Zendy; N.C. Helman | Zendy; Wendell A. Lim | Zendy; Paul J. Hung | Zendy

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A Microfluidic System for Dynamic Yeast Cell Imaging

Author(s) -

Philip Lee,

N.C. Helman,

Wendell A. Lim,

Paul J. Hung

Publication year - 2008

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112673

Subject(s) - microfluidics , saccharomyces cerevisiae , yeast , live cell imaging , microbiology and biotechnology , fluorescence lifetime imaging microscopy , cell , biology , green fluorescent protein , computational biology , nanotechnology , gene , fluorescence , biochemistry , materials science , optics , physics

The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae.

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