A Method for the Rapid Isolation of Virus from Cultured Cells | Zendy

ByungWhi Kong | Zendy; Linda K. Foster | Zendy; Douglas N. Foster | Zendy

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A Method for the Rapid Isolation of Virus from Cultured Cells

Author(s) -

ByungWhi Kong,

Linda K. Foster,

Douglas N. Foster

Publication year - 2008

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112589

Subject(s) - lysis , distilled water , virology , virus , biology , metapneumovirus , isolation (microbiology) , cell culture , microbiology and biotechnology , chemistry , chromatography , human metapneumovirus , respiratory system , respiratory tract infections , genetics , anatomy

A simple and efficient collection method using hypotonic burst to isolate virions from infected cultured cells is described. Distilled water treatment of avian metapneumovirus (AMPV)-infected cells with thorough mixing and repeated pipeting was considerably faster for virion collection in avian cells compared to the widely used freeze-thaw (F-T) method (30 min vs. 3-4 h). This method was also more effective for virion collection. The total number of virions recovered from AMPV-infected immortal turkey turbinate cells by the novel water lysis method was 3-fold higher than by the F-T method. This simple water lysis method can be applied to virion collection for other RNA viruses such as the paramyxoviruses that are used to infect cultured cells.

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