Improved Recombinational Stability of Lentiviral Expression Vectors using Reduced-Genome Escherichia Coli | Zendy

Chacko S. Chakiath | Zendy; Dominic Esposito | Zendy

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Improved Recombinational Stability of Lentiviral Expression Vectors using Reduced-Genome Escherichia Coli

Author(s) -

Chacko S. Chakiath,

Dominic Esposito

Publication year - 2007

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112585

Subject(s) - escherichia coli , cloning (programming) , biology , genome , genetics , strain (injury) , computational biology , gene , computer science , anatomy , programming language

Lentiviral expression clones, which contain long direct repeats, often show dramatic instability in Escherichia coli, leading to difficulties in obtaining valid clones. We show that the reduced-genome E. coli strain MDS42 is capable of stabilizing lentiviral expression clones containing direct repeats, and outperforms many commonly used cloning strains for this purpose. In addition, the strain has several characteristics that make it highly amenable for use in recombinational cloning systems.

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