Epifluorescent Microscopy to Evaluate Bacteriophage Capsid Integrity | Zendy

Kerusha Naicker | Zendy; Steven I. Durbach | Zendy

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Epifluorescent Microscopy to Evaluate Bacteriophage Capsid Integrity

Author(s) -

Kerusha Naicker,

Steven I. Durbach

Publication year - 2007

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112584

Subject(s) - capsid , nucleic acid , dna , bacteriophage , bacteriophage ms2 , microscopy , biology , dna extraction , microbiology and biotechnology , chemistry , virus , virology , polymerase chain reaction , biochemistry , escherichia coli , gene , physics , optics

The standard method to evaluate capsid integrity of DNA-based viruses, which depends on access to DNase I, relies on the removal of the capsid by solvent extraction and then the evaluation of the nucleic acid products by electrophoresis. Our method, which is based on the direct detection of capsid-borne DNA directly through the use of epifluorescent microscopy, negates the requirement of DNA extraction.

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